In a world where toys are living things who pretend to be lifeless when humans are present, a group of toys, owned by six-year-old Andy Davis, are caught off-guard when Andy's birthday party is moved up a week, as Andy, his single mother and infant sister Molly are preparing to move the following week. The toys' leader and Andy's favorite toy, an old-fashioned cowboy doll named Sheriff Woody organizes the other toys, including Bo Peep the shepherdess, Mr. Potato Head, Rex the Dinosaur, Hamm the Piggy Bank and Slinky Dog, into a scouting mission. Green army men, led by Sarge, spy on the party and report the results to the others via baby monitors. The toys are relieved when the party appears to end with none of them having been replaced, but then Andy receives a surprise gift – an electronic toy space ranger action figure named Buzz Lightyear, who believes that he is an actual space ranger.
Buzz impresses the other toys with his various features, and Andy begins to favor him, making Woody feel left out. As Andy prepares for a family outing at Pizza Planet, his mother allows him to bring only one toy along. Fearing Andy will choose Buzz, Woody attempts to trap him behind a desk, but ends up knocking him out a window instead, resulting in the other toys accusing Woody of murdering Buzz out of jealousy. Before they can exact punishment, Andy takes Woody instead and leaves for Pizza Planet. When the family stops for gas, Woody finds that Buzz has hitched a ride on the car as well, and the two fight, only to find the family has left without them. They manage to make their way to the restaurant by stowing away on a pizza delivery truck, where Buzz, still believing he is a real space ranger despite Woody's attempts to convince him otherwise, gets them stuck in a crane game, where they are picked out by Andy's destructive neighbor Sid Phillips.
Woody attempts to escape from Sid's house, but Buzz, finally discovering he is a toy, sinks into despondency. Sid plans to launch Buzz on a firework rocket, but his plans are delayed by a thunderstorm. Woody tells Buzz about the joy he can bring to Andy as a toy, restoring his confidence. The next morning, Woody and Sid's mutant toy creations rescue Buzz just as Sid is about to launch the rocket and scare Sid into no longer abusing toys by coming to life in front of him. Woody and Buzz then leave Sid's house just as Andy and his family drive away toward their new home.
The duo tries to make it to the moving truck, but Sid's dog, Scud, sees them and gives chase. Buzz gets left behind, and Woody tries rescuing him with Andy's RC car, but the other toys, thinking Woody eliminated RC as well, attack and toss him off the truck. Having evaded Scud, Buzz and RC pick up Woody and continue after the truck. Upon seeing Woody and Buzz together on RC, the other toys realize their mistake and try to help them get back aboard but RC's batteries become depleted, stranding them. Woody ignites the rocket on Buzz's back and manages to throw RC into the truck before they soar into the air. Buzz opens his wings to free himself from the rocket before it explodes, gliding with Woody to land safely into a box in the van, right next to Andy.
On Christmas Day, at their new house, Woody and Buzz stage another reconnaissance mission to prepare for the new toy arrivals. As Woody jokingly asks what might be worse than Buzz, they discover Andy's new gift is a puppy, and the two share a worried smile.
- The storyline continues in Brian and the Eeveelution Family's Adventures of Toy Story 2.
- Opening/"You've Got a Friend in Me"
- The staff meeting/the birthday party
- Enter Buzz Lightyear/"Strange Things"
- Woody and Buzz's argument/Sid Phillips
- Woody sabotages Buzz
- At the gas station/Woody vs. Buzz
- I'm lost!/The Pizza Planet truck
- At Pizza Planet/captured by Sid/Woody's gone
- Playtime with Sid/Buzz discovers the truth/"I Will Go Sailing No More"
- I am Mrs. Nesbitt!
- Sid to Andy's window/Buzz repair
- The Big One
- Buzz and Woody talk/Operation: Rescue Buzz!
- The plan in action
- Play nice
- Catching up to the moving van
- ROCKET POWER!!!!
- Christmas day/Happy ending