Eeyore (voiced by Peter Cullen) is a gloomy Donkey who is depresed all the time and he is one of Pooh's good friends. He lives in a short house made from sticks in a place called Pooh Corner in the 100 Acre Wood. His house is sometimes wrecked and he always fixes it.
- Eeyore, alongside Pooh, Piglet, Tigger and Rabbit will make their first appearance in Team Robot's Adventures of Barney's Imagination Island and will join be guest starring In Team Robot & Mr. Conductor's Adventures Of Timmy The Tooth
- Eeyore is a good friend of Strudel, and cousin of Shrek's Donkey.
- Eeyore was also voiced by the late Bud Luckey in the 2011 film and by Brad Garrett in a few Winnie the Pooh computer games (including the Winnie the Pooh and the Honey Tree Animated Storybook, Ready to Read with Pooh, and Ready for Math with Pooh) and will reprise his role in the live-action film Christopher Robin.