Eeyore (voiced by Peter Cullen) is a gloomy Donkey who is depresed all the time and he is one of Pooh's good friends. He lives in a short house made from sticks in a place called Pooh Corner in the 100 Acre Wood. His house is sometimes wrecked and he always fixes it.
- Eeyore, alongside Pooh, Piglet, Tigger and Rabbit will make their first appearance in Team Robot's Adventures of Barney's Imagination Island and will join be guest starring In Team Robot & Mr. Conductor's Adventures Of Timmy The Tooth
- Eeyore is a good friend of Strudel, and cousin of Shrek's Donkey.