In the film, Gladys is disgusted by animals and is very strict on her rules. She hires Dwayne to exterminate the animals, to no avail. In the end, Gladys gets fired from her job and arrested for illegal traps after trying to kill RJ and the gang with her weed hacker.
Gladys is cruel, short-tempered, greedy, violent, heartless, psychotic, and black-hearted. She is a wild animal hater and hires Dwayne to take out RJ and his friends.
Gladys is greedily obsessed with all of the homes in the area being perfect and wants to get rid of the animals because she is not only disgusted and horrified by them, but also thinks they are lowering the property values.
Gladys wears a grey dress suit. She has pale skin and shortish black hair.
After she was hit by the Depelter Turbo, she lost all her precious hair and became bald.
- Gladys is similar to Cruella de Vil: Both are animal haters and get arrested at the end of the film. The only difference between the two is that Cruella De Vil is the main antagonist of her film (101 Dalmatians) and Gladys is the secondary antagonist of her film (Over the Hedge).
- She's voiced by Allison Janney, who also does Mrs. Grunion, whose character has a bit similarity to hers.