Holley Shiftwell is the secondary tritagonist in Cars 2. She is a beautiful young British desk agent turned spy-in-training who is stationed in Tokyo. Well-educated and sharp, she knows every trick in the book (or rather, she relies on every trick in the spy manual). She is armed with all of the latest state-of-the-art spy equipment imaginable, from hidden cameras and concealed weapons to a telescoping utility arm and a holographic pop-up display. Holley is a highly motivated agent, but is fresh out of the academy, so her experience is based on lessons learned in school rather than real-life situations. When Finn McMissile requires Holley's technical expertise for his latest top-secret field operation, she finds herself pursuing a rendezvous with the most unlikely candidate -- Mater, an innocent tow truck caught up in the intrigue, who is mesmerized by the beauty of his newest friend.
- Holley was voiced by Emily Mortimer.
- Holley Shiftwell will meet Winnie the Pooh, Princess Twilight Sparkle, Ash Ketchum, Littlefoot, Alex, Skipper, SpongeBob, Tommy and their friends in Pooh's Adventures of Cars 2.
- Holley Shiftwell will meet Ryan, Crash, Thomas and their friends in Ryan's, Thomas' and Crash's Adventures of Cars 2.