Mario Mario, famous mascot of Nintendo and widely recognized as the face of video gaming in general, is a Brooklyn-born Italian plumber residing in the Mushroom Kingdom, whom Pooh meets in Pooh's Adventures of Super Mario Sunshine, along with his brother, Luigi. In his games, Mario is known for being able to use a wide variety of power up items to enhance his abilities, such as Fire Flowers, Super Mushrooms, Power Stars, among others. This is a trait he shares with Luigi, despite the latter being more timid and wary. They have many friends in the Kingdom, namely Yoshi, a green dinosaur with a huge appetite and prehensile tongue, the numerous Toads which inhabit the kingdom, and Princess Peach Toadstool, the fair and beautiful ruler who shares a romantic interest with Mario. However, Mario also has his share of enemies, most prominently Bowser, the wicked King of the Koopas. He is the adopted father of Lilo and Nani Pelekai.
- Mario will meet Littlefoot and his friends in Pooh's Adventures of Chaos Emerald hunt.
- Mario will see Littlefoot and his friends again in Winnie the Pooh and Kirby Right Back at Ya.
- Mario will meet Mordecai, Rigby, T-alan, and George Volcano in Winnie the Pooh goes to Hotel Mario.
- Mr. Conductor and his friends meet Mario in Mr. Conductor's Adventures in The Super Mario Bros. Super Show
- Mario and Peach adopted Lilo and Nani, making them their daughters and Luigi and Daisy's nieces at the same time adopting Stitch, Angel and Scorpio as their pets.
- It is believed that Rosalina is Peach's sister.