Marlene is a female otter on The Penguins of Madagascar.
- Marlene will make her first guest appearance in a Lion King crossover film in Simba, Timon, and Pumbaa's Adventures of Enchanted.
- Marlene will make her first guest appearance in a Land Before Time crossover film and meet Littlefoot and his friends in Littlefoot's Adventures of Love and the Happy Days.
- Marlene became Timon's Wife.
- Marlene is voiced by Nicole Sullivan who also voices Shego in Disney's Kim Possible.