Marshal Moo Montana, The Dakota Dude and Cowlorado Kid are the main heroes in Wild West C.O.W. Boys of Moo Mesa.
- Marshal Moo Montana is the leader of the C.O.W.-Boys and marshal of Moo Mesa. Courageous and quick on his hooves, Moo Montana "battles the bad guys and makes the West a safer place to graze".
- The Dakota Dude is the soft-spoken muscle of Montana's posse, Dakota possesses a cool temperament and rarely gets mad, even in near-death experiences.
- Cowlorado Kid is a Holstein cattle who is the youngest of the group and a self-proclaimed ladies' man with a good singing voice, regardless of his skill with the lasso and guitar.
- They will meet Winnie the Pooh, Ash Ketchum and friends in Pooh's Adventures of Wild West C.O.W. Boys of Moo Mesa.
- They will join the team in Winnie the Pooh Meets Calamity Jane.