Otis is a care-free cow who becomes the leader of the barnyard after the death of his father, Ben in Barnyard.
- Otis met Simba and his friends in Simba, Timon, and Pumbaa's Adventures of Barnyard: The Original Party Animals.
- Otis will meet Winnie the Pooh and his friends in Winnie the Pooh Goes to Barnyard: The Original Party Animals.
- He'll start his own Adventures.
- Otis will meet Bloom and her friends in Bloom Goes to Barnyard: The Original Party Animals.
- He was voiced by Kevin James, who also voiced the Hotel Transylvania version of Frankenstein, in the movie and Chris Hardwick in the series Back at the Barnyard.