Three weeks before the Indominus rex Paddock became open to the public, he was offered a job by Claire Dearing to check the paddock for any security breaches. It is unknown if he accepted the job. Some time afterward the remaining Indominus rex escaped her paddock and went on a rampage. He went to investigate the paddock after her escape with a Timack Construction worker and a paddock supervisor only for the Indominus rex herself to appear and attack them. She killed the construction worker, but Owen made it out alive by exiting through her paddock's steel doors and hiding under an automobile just before she broke through the doors He then proceeded to cover his scent by cutting the car's gas tank.
Owen took part in an attempt to end the hybrid's rampage. A notable action of Owen during this time was investigating a damaged Gyrosphere with Claire Dearing and tending to an injured Apatosaurus with her as well. Owen also had his Velociraptor pack assist him in his pursuit of the Indominus rex. At some point, he witnessed the inhabitants of the Isla Nublar Aviary's escape and invasion of Main Street. After the flocks passed through Indominus rex arrived in Main Street. During I.rex's time in the street, Owen defended Gray and Zach, the nephews of Claire Dearing. 
At some point in their lives, Owen and Claire once went on a date. As Owen states, she was too demanding and uptight and it was this reason why he never continued to date her. But he still seems to be interested in her as he attempted to flirt with her when she traveled to his bungalow to offer him a job.
While Vic Hoskins believes that by controlling the raptors, they can use them as weapons, Owen held his belief that raptors are never to be controlled and forbids Vic to let the raptors out. But when Masrani was kiled, their rivalry grew as Hoskins gains control and orders Owen to let the raptors loose. He was even more outradged when he learns that Hokins plans to use the dinosaur embryos from Henry Wu's lab to make new dinosaurs for war combat. According to Hoskins' Lego profile, he secretly wants to steal Owen's research to make a profit.
- Owen Grady was played by Chris Pratt who also voiced Emmet and played Peter Quill (Star-Lord).
- Owen Grady will meet Winnie the Pooh, Ash Ketchum, Littlefoot, Simba, SpongeBob SquarePants, and their friends in the sixth season of Pooh's Adventures of Once Upon a Time.
- Owen Grady will see Pooh, SpongeBob, Ash, Littlefoot, Simba, and their friends again in Winnie the Pooh and SpongeBob SquarePants Go to Jurassic World.
- Owen Grady will meet Thomas, Twilight Sparkle and their friends in Thomas and Twilight Sparkle visit Jurassic World.
- Owen Grady will meet Tino Tonitini in Weekenders visits Jurassic World.
- Owen Grady will meet Roary, Theodore and their friends in Roary and Theodore visit Jurassic World.
- Owen Grady will meet Heckle, Jeckle and Cool McCool in Heckle, Jeckle and Cool McCool's Adventures of Jurassic World.