The Ponyville Spa was first featured in Bridle Gossip, is apparently run by the spa ponies, one of whom, Lotus Blossom, speaks with an accent. The spa can be seen during long shots as being on the perimeter of the town square, next to the joke shop. In Feeling Pinkie Keen
, ponies are seen having their own bath tubs. In Bridle Gossip, Lotus Blossom asks Zecora for her recipe for a bath.
At the beginning of Green Isn't Your Color, Rarity apologizes to Fluttershy for being late to one of their weekly get-togethers, and requests "the usual" regimen from the Spa ponies. The spa makes a brief appearance in A Friend in Deed, when Pinkie Pie brings Cranky Doodle Donkey for a makeover in an attempt to win his friendship. In Ponyville Confidential, the six main ponies and Spike are at the spa when they discuss the latest "Gabby Gums" column in the Foal Free Press.
Twilight and Spike spend a day at the spa in Castle Sweet Castle, while Twilight's friends redecorate her castle.