Scarlet Overkill (voiced by Sandra Bullock) is an up-and-coming super-villain from the Despicable Me prequel Minions who lives in the late 1960's. She is the world's first major female super-villain, and often works alongside her inventor husband, Herb. Her major gadget is her red dress, which can turn into a rocket and has several built-in weapons. She plans to steal Queen Elizabeth II's crown so she can rule all of England. She hires Kevin, Stuart, and Bob to help her carry out her plan.
- Scarlet will become Winnie the Pooh's new enemy in Pooh's Adventures of Minions.
- Scarlet will make her first guest appearance in a Winnie the Pooh/Pokémon mixed crossover film to get her revenge on Pooh, Ash, and their friends in Pooh and Ash's Adventures of Scooby-Doo: Wedding Bell Boos.
- Scarlet will make her first guest appearance in a solo Winnie the Pooh crossover film to get her revenge on Pooh and his friends in Pooh's Adventures of Scooby-Doo and the Ghoul School.
- Scarlet will become Littlefoot's new enemy in Littlefoot's Adventures of Minions.
- Scarlet will become Tino's new enemy in Weekenders Adventures of Minions.
- Scarlet will make her first guest appearance in a Dinosaur crossover film to get her revenge on Aladar and his family in Aladar's Adventures of Scooby-Doo on Zombie Island.
- Scarlet will become Roary and Theodore's new enemy in Roary and Theodore's Adventures with the Minions.