Simba, Nala, Timon, Pumbaa, and the Jungle Adventure crew (along with Alex, Marty, Melman, Gloria, the Penguins, Po the Panda, and the Furious Five) have traveled to Hollywood in the year 1947 and meet L.A.'s number 1 Toon star named Roger Rabbit and they help him along with A Private Toon Investigator Named Eddie Valiant to solve the mystery of the murder of Marvin Acme and save ToonTown before it goes into the hands of Judge Doom (whom the Jungle Cubs Vultures and Tai Lung work for).
- Alex, Marty, Melman, Gloria, The Penguins of Madagascar, Po the Panda, The Furious Five, Arthur and Cecil, and Tai Lung will guest star in this film.
- This is an NTSC film with NTSC bits from The Lion King films, the Madgascar films, Tarzan, The Princess and the Frog, the Aladdin film, The Little Mermaid films, The Jungle Book, TaleSpin: Plunder and Lightning, Brother Bear 1 and 2, Rock-a-Doodle, and Kung Fu Panda 1 and 2.
- Genie has met Roger Rabbit before in Ash Ketchum Meets Tom Sawyer.
- Timon, Pumbaa, Genie, Trek, Tantor, Alex, Marty, Melman, Gloria, The Penguins Of Madagascar, King Julien, Maurice, and Mort will see Roger Rabbit again in Winnie the Pooh in Animagique.
- This film will be dedicated in memory of Bob Hoskins (who played Eddie Valiant) who died from pneumonia on April 29, 2014.
- Jonathan Oosterhof (aka LionKingRulezAgain1) was originally going to make this film, but he retired from making anymore crossovers, so Yakko Warner will make this film instead.