Marshal Moo Montana: Leader of the C.O.W.-Boys and marshall of Moo Mesa. Courageous and quick on his hooves, Moo Montana "battles the bad guys and makes the West a safer place to graze". Lives by the Code of the West, which he seems to make up as he goes along. His horse's name is Cyclone.
The Dakota Dude: The soft-spoken muscle of Montana's posse, Dakota possesses a cool temperament and rarely gets mad, even in near-death experiences. His horse's name in Rebel and is scared of heights.
The Cowlorado Kid: The youngest of the group and a self-proclaimed ladies' man with a good singing voice, regardless of his skill with the lasso and guitar. Cowlorado isn't a deputy yet. His horse's name is Jesibelle.
- The C.O.W. Boys of Moo Mesa will appear in Untitled Wild West Crossover Story where they become allies of the Trimaxian Adventure Crew.