The Cutie Mark Crusaders (Apple Bloom, Scootaloo, and Sweetie Belle) are young ponies who will make their first appearance in the second series of Thomas and Twilight Sparkle's Adventures. After they finally earned their cutie marks, they are now ponies on a mission to help other ponies find their special talents from their cutie marks.
- Apple Bloom is Applejack and Big McIntosh's younger sister.
- Sweetie Belle is Rarity's younger sister.
- Scootaloo is Rainbow Dash's biggest fan and adopted younger sister.
They will join the team in Thomas the Tank Engine Meets Dumbo.
- The Crusaders met Pooh and his friends in Pooh's Adventures of My Little Pony: Friendship is Magic (Full Movie)
- The Crusaders met the FT Squad in The FT Squad's Adventures in My Little Pony: Friendship is Magic.
- They are also friends with Nanako Dojima in Yu Narukami and Aigis's adventures My Little Pony Friendship is Magic.
- They also own a train.
- They also own a 38 revolver, .9 ml pistol, and a Desert Eagle.
- They are also great friends with the Jelly Bean Scouts, Lazlo, Raj, and Clam. The six of them form a team called the Jelly Bean Crusaders in Dipper & Mabel's Adventures Chronicles.
- The Cutie Mark Crusaders will make their first appearance in Weekenders Adventures of The Land Before Time II: The Great Valley Adventure and other projects.
- The Cutie Mark Crusaders are best friends with Lilo, Stitch, Angel (Lilo & Stitch), Cream the Rabbit and the Powerpuff Girls.
- The Cutie Mark Crusaders are best friends with Peter Sam, Sir Handel, and Duncan.
- They are friends of Super Secret Pup Club (Rebound, Cupcake, and Patches).
- They are also best friends with Dusty Crophopper.
- The Cutie Mark Crusaders will guest star in Pooh's Adventures of Fantastic Four (2005).
- The Cutie Mark Crusaders will make their first appearance in Team Robot's Adventures of Barney's Imagination Island.
- In the series, Power Rangers Data Squad, Sweetie Belle will fall in love with him for when he cures her from Eggman's Love Potion.
- Sweetie Belle will play the role of Pepper Potts in My Little Pony: Iron Mare, My Little Pony: Iron Mare 2, My Little Pony: The Harmony Avengers, and My Little Pony: Iron Mare 3.