The Matrix of Leadership also appears in the Transformers film sequel Transformers: Revenge of the Fallen. It is introduced into the film when the Fallen uses it to activate the Sun Harvester hidden in Cairo, Egypt. The original Primes had hidden it there, sacrificing their own lives to protect humanity from the power of the Harvester. The appearance of the Matrix is similar to that of the original G1 appearance, but the "handle-bar" is more triangular and is striped. A small energy ball is encased by a netted metal cage (spherical) in the middle.
Sam Witwicky, joined by Mikaela Banes, Leo Spitz, Seymour Simmons, Skids, Mudflap and Bumblebee, search through a temple for the Matrix and find it surrounded by the bodies of the original Primes. When he holds it in his hands, it turns to sand and he later discovers it has to be earned and not given. It is earned by Sam's sacrifice for Prime, and is used to revive Optimus Prime after the attacks of Megatron and his minions. It is then stolen by the Fallen and used to power the Solar Harvester, but is apparently recovered after the Fallen's death and the destruction of the Harvester.
- Thomas, Twilight Sparkle, and their friends will see the Matrix in Thomas and Twilight Sparkle's Adventures of Transformers Revenge of the Fallen.