Maggie is the only cow left on the Dixon Ranch after Alamida Slim (a cattle rustler capable of stealing 500 in a single night) stole all the rest of Dixon's cattle. Mr. Dixon sells Maggie to Pearl, a kind and elderly woman that runs a small farm called Patch of Heaven.
The local Sheriff arrives to tell Pearl that her bank is cracking down on debtors. Pearl has three days to pay the bank $750, or her farm will be sold to the highest bidder. Hearing this, Maggie convinces the other cows on the farm (Grace, a happy-go-lucky character, and Mrs. Calloway, who has had leadership gone to her head) to go to town to attempt winning prize money at a fair. And Thomas, Twilight Sparkle and their friends come along too.
While the cows are in town, a bounty hunter named Rico (who Buck, the Sheriff's horse, and Applejack and Apple Bloom's cousin, idolizes) drops a criminal off and collects the reward. And Steamy, Puffy, and Shai-Shay reunite with our heroes and tell them that they're here to settle a score with Rattlesnake Jake. Stating he needs a replacement horse to go after Alamida Slim while his own horse rests, he takes Buck. When Maggie find out that the reward for capturing Slim is of exactly $750, she convinces the other cows to try to capture him to save Patch of Heaven.
That night, they hide among a large heard of steers, when Alamida Slim and Jake appears. Before any of them can do anything, Slim begins a yodeling song which sends all the cattle, Pinkie Pie, Rarity, and Fluttershy (except Grace, who is tone deaf) into a trance that causes them to dance madly and follow Slim anywhere. Grace and our heroes are able to bring Maggie, Mrs. Calloway, Pinkie Pie, Rarity, and Fluttershy back to their senses just before Slim closes the path behind him with a rockslide to stop Rico and his men from chasing him.
As Rico discusses with his men what his next move will be, Buck starts talking with Maggie, Grace, Mrs. Calloway, and our Heroes as old friends and miming actions. This causes Rico to believe Buck is frightened by cows, talking trains, and ponies, and sends Buck back to the Sheriff. Buck escapes, determined to capture Slim for himself to prove his worth. Maggie, Grace, and Mrs. Calloway and our heores are left behind, then set off again. But that night, a rainstorm happens and causes a flash flood sending a gaint tidal wave towards the posse, the trains brake hard and put it in full reverse but due to the rails being slick from rain they are engulfed by the wave. The cows swim to a small rock island, while our heroes struggle to get there but have trouble in the current and the other engines get stuck in their spots. Steamy, Puffy, and Shai-Shay then use their logging strength to get them to the island and save Rarity (who doesn't know how to swim) from drowning, Mrs. Caloway then decides to give up and head home tommorow morning and insults Maggie. Our heroes stand up for Maggie and scold Mrs. Caloway, (Gordon stating he knows vulgur when he sees it and Maggie's tricks are not) Steamy then adds that she'll die before she makes it back which gets Caloway thinking. The next morning they meet a peg-legged rabbit named Lucky Jack, who leads them to the Slim's hideout mine.
At the mine, Slim reveals that he has been stealing all cattle from his former patrons. When his former patrons can't support their land anymore, he buys the land when it is auctioned off, under the guise of the respectable-looking Mr. O'delay, using the very money he gets from selling the cattle he stole.
After arriving to Slim's hideout, the cows and our heroes capture Slim. They run off with Slim's accomplices, Jake and buyer in pursuit on a steam train. Rico arrives. When the chase stops, Rico is revealed to work for Slim.
Slim dons his O'delay costume and leaves the cows and our heroes stranded in the middle of the desert with the train, while he goes to attend the auction with Jake right behind him. Then Buck and our heroes battle the buyer, Willie boys, and Rico and Steamy, Puffy, and Shai-Shay use their guns to shoot them. After that the cows and our heores arrive using the train to the farm and expose Slim. Then Jake, Steamy, Puffy, and Shai-Shay have a showdown and get Jake distracted to use up all of his anmo, Chrysalis appears out of nowhere and swips Shai-Shay's revolver to shoot Jake but it's empty! Steamy takes his twin barrel 12-gauge and shoots Chrysalis in the legs then Jake gets up and sees Shai-Shay emptied his revolver of it's only bullet. Jakes thanks Shai-Shay of his deed and tells the locos he is in their debt, then Jake says to Chrysalis: "Pretty soon, no one will believe you even existed." Then he wraps his coils around the changling queen and takes her away. Slim is arrested by the police, and Patch of Heaven is saved by the reward money.
- It is revealed that Buck is Applejack's and Apple Bloom's older cousin.
- Rattlesnake Jake will work for Alameda Slim in this film.
- Queen Chrysalis will make a climax appearance in this film.
- Duke, Steamy, Puffy, and Shai-Shay will guest star in this movie.
- When Alameda Slim does his yodeling, Pinkie Pie, Rarity, and Fluttershy will get hypnotized too.
- It's revealed that Rarity has huge Ophidiophobia: "a fear of snakes" and can't swim.
- Part 1: https://plus.google.com/photos/100495329807899486850/albums/6137717009450548705/6137717010753830770?pid=6137717010753830770&oid=100495329807899486850
- Part 2: https://plus.google.com/photos/100495329807899486850/albums/6137717009450548705/6137724574893008082?pid=6137724574893008082&oid=100495329807899486850
- Part 3: https://plus.google.com/photos/100495329807899486850/albums/6137717009450548705/6137780930022219746?pid=6137780930022219746&oid=100495329807899486850
- Part 4:https://plus.google.com/photos/100495329807899486850/albums/6137717009450548705/6213859334319296130?pid=6213859334319296130&oid=100495329807899486850
- Part 5: https://plus.google.com/photos/100495329807899486850/albums/6137717009450548705/6217482141384616386?pid=6217482141384616386&oid=100495329807899486850
- Part 6: https://plus.google.com/photos/100495329807899486850/albums/6137717009450548705/6226511723178837938?pid=6226511723178837938&oid=100495329807899486850
- Part 7: https://plus.google.com/photos/100495329807899486850/albums/6137717009450548705/6238722529562383106?pid=6238722529562383106&oid=100495329807899486850
- Part 8 (final part): https://plus.google.com/photos/100495329807899486850/albums/6137717009450548705/6238735214830600610?pid=6238735214830600610&oid=100495329807899486850