This is how Twilight loses hope goes in The Return of Harmony Part 2.
[Twilight looks at Optimus]
Midnight: Well, well, well, I see you've found the Elements of Harmony. How terrifying!
Twilight Sparkle: Midnight! I've figured out your lame riddle. You're in for it now!
Optimus Prime: Yeah.
Midnight: [sarcastically] I certainly am. You've clearly out-dueled me, and now it's time to meet my fate. I'm prepared to be defeated now, ladies and Autobots. Fire when ready.
Twilight Sparkle: Formation, now!
[Our heroes get into persition]
Twilight Sparkle: All right, let's get this over with.
[The Elements start to power up as Midnight watches and the heroes prepare to fire when Twilight thinks about what Midnight says]
Midnight: (voice) You fail them all if you cannot change.
[Twilight then takes off the Element of Magic, cancelling the attack]
Rainbow Dash: What's going on?
Arcee: I don't understand.
Applejack: Mine's working. There must be something wrong with them.
Builkhead: I can't see anything wrong.
Pinkie Pie: Why'd you do that, Twilight?
Smokescreen: What happened?
Fluttershy: Well, that was weird.
Ratchet: I can tell.
Rarity: What happened?
Bumblebee: [beeps] (translation: I don't know.)
Fluttershy: Well, what it is, we'll find out soon.
Optimus Prime: Twilight, what is the matter?
Twilight Sparkle: He's right.
Midnight: Bravo, Ponies and Autobots, bravo. Harmony in Equestria is officially dead. Midnight rules, Celestia drools. Unicron wins, Celestia loses. [laughing]
[Midnight leaves to continue his chaos. Twilight grits her teeth]
Pinkie Pie: What's wrong, Twilight?
Twilight Sparkle: He's right.
Pinkie Pie: What?
Applejack: Are y'all saying we've met our match?
Fluttershy: That can't be true.
Rarity: It just can't be.
Rainbow Dash: Seriously? We've... met our match?
Smokescreen: We can't just give up and let an apprentice of Unicron win.
Ratchet: Come on.
Bumblebee: [beeps] (translation: Pull it together.)
Arcee: We can't give up now.
Optimus Prime: They are right, Twilight.
Twilight Sparkle: But if he is saying is true? Maybe we couldn't have done it anyway.
[Twilight's colors fade and Twilight sheds a tear that splatters in the shape of a broken heart. Twilight then walks away from her friends. They watch as she vanishes over the horizon]
Rainbow Dash: I wish there was something we could do to cheer her up.
Pinkie Pie: Me too.
Smokescreen: You and me both.
Rarity: Bee, what do you think?
Bumblebee: [beeps] (translation: I think we should.)
Applejack: He's right, ladies.
Fluttershy: Let's do it.
Ratchet: I agree.
Optimus Prime: Autobots, Equines, roll out!
[Twilight walks through Ponyville when Midnight appears]
Midnight: Twilight, you've got to see what I just did.
[He leads her to her castle]
Midnight: It's priceless! [laughs]
[Midnight sees Twilight is sad]
Midnight: Come now, Twilight Sparkle. You've got to get into the spirit of things. [touches her shoulder] After all, this is your new home.
[Twilight shakes her head]
Twilight Sparkle: Not anymore.
[Twilight is about to walk away]
Midnight: Twilight, you mean everything to me.
Twilight Sparkle: Really?