The movie begins with "non-copyright-infringing movie parodies", including parodies of Star Wars (with distinct mockeries of Darth Vader and Jar Jar Binks), Spider-Man, and Jurassic Park, all with the villains of the parodies saying, "Tell me your secret, Timmy Turner!" presumably meaning his fairy godparents' existence. After getting swallowed whole by a dinosaur, Timmy wakes up, finding it all to be a dream - enabling Cosmo and Wanda to prepare him for his Fairy-versary party. Among the party goers are the Tooth Fairy, the Easter Bunny, Baby New Year, the April Fool, Santa Claus, Jeff the Elf, Cupid, and Jorgen Von Strangle. An anniversary song is sung to Timmy by Fairy Cowlick Jr., and he is given a variety of magical gifts which will come into important play later on.
Cosmo and Wanda explain that a Fairy-versary is the day that celebrates having kept his fairies a secret for one year. Timmy asks if that is a big deal. The Easter Bunny says it is because by this time most godchildren would have already had their fairy godparents taken away. Timmy asks why and Jorgen shows an example explanation. According to Jorgen, usually by one year a godchild has done something to reveal the existence of their fairies. The party is kept uninterrupted by a "Forget-me-Knob" installed in the door, which when touched, immediately causes the person opening the door to forget what they were doing. Among the various magical gifts Timmy receives from the magical friends he has made over the past year, that includes a jetpack, floss, glasses that changes faces, arrow and bow, a mirror, grenade-like Easter eggs and Santa's magic bag, he is given a magical (but bad-tasting) muffin, which can grant a rule-free wish. Timmy asks how he got fairy godparents and Wanda tells him the story. A flashback is shown with 8-year-old Timmy and how a 14 year old Vicky got into his life and started torturing him. Timmy realizes he is miserable because his parents lied to him and tricked him into having Vicky hired.
Timmy inadvertently wishes for his parents to always tell the truth, and when he questions his parents why they lie to him, they give him the harsh truth. The school bus arrives and they shoo him off to school, but Mr. and Mrs. Turner are noticeably bothered when they start to count the amount of times they lied to Timmy and it begins to number in the thousands. Meanwhile at Dimmsdale Elementary School, Timmy takes the magic muffin to school with him. Mr. Crocker, whom had been stalking Timmy in his "Inconspicuous" Van, detects Fairy Magic in the shape of a muffin and fires a fork at Timmy's lunch bag. The fork pierces the bag and causes the muffin to fall away from Timmy, and then the fork gets embedded in Principal Waxelplax's rear end. Mr. Crocker reels in Waxelplax like a fish only to realize his mistake, and try to cover himself by saying he was in love with Waxelplax, which she finds sudden and unwanted.
Timmy notices the muffin is gone and he tries everything to get it back. Inside Mr. Crocker's class, Crocker is so distracted by the muffin he cannot even teach, and tells the children to stare at the black board until lunch time. As the bell rings, Timmy realizes he has been chained to his desk, and Crocker tells Timmy his plans to get the muffin before Timmy does and use its magic to rule the world. Timmy wishes himself free of the chains and reaches the lunch room, only to discover at lunchtime that today is "Muffin Monday" and loses it among mountains of muffins. As Crocker has a magic detecting device with him, Timmy devises a way to slow him down by starting a muffin food fight. Elsewhere in the cafeteria, a monkey named Bippy belong to A.J. is caged and watching the food fight. Timmy feels sorry for him and lets him out. However, as Timmy, as well as Mr. Crocker, continue to search for the muffin, Bippy finds the muffin before either one of them, and takes a bite. The monkey makes a wish, and since anyone can use the magic muffin, his wish is granted.
Suddenly, the whole world is transformed, leaving monkeys the rulers of Earth. To make matters worse, Timmy loses his Godparents when Jorgen von Strangle announces to all fairies that Da Rules say they have to be Godparents of the "dominant species" on Earth: monkeys. Bippy ends up getting Cosmo and Wanda as godparents, and is for a time apparently the subject of his own Fairly OddParents opening song spoof (with heavy emphasis on bananas).
Timmy, who still remembers Cosmo and Wanda, follows Bippy to try to get him to unwish everything. Timmy returns to Mr. Crocker's class, who is now ranting about Apes using fairies to take over the world. Mr. Crocker is taken by the apes to a place off-limits to Humans. With Crocker gone, Timmy enters the Crocker Cave and steals a magic detector. He uses the magic detector to track down Bippy, whom he finds with the Fairy-versary muffin along with Cosmo and Wanda. Timmy almost succeeds in retrieving the muffin, but is captured by the Apes and brought to the human testing center along with Mr. Crocker and Francis. Crocker is to be dissected first, but he fearfully begs for Timmy to be taken instead. Timmy is taken to a surgical room where his skull is about to be drilled into when Bippy showed up to rescue him. Bippy manages to fight off the apes at first, but is eventually overwhelmed.
Luckily, due to Cosmo's 'paraphrasing' of Bippy's wish, which Cosmo claims could just as easily have been for another banana, everything is unwished moments before Timmy was about to be harmed. Unfortunately, Bippy also lost the muffin during the scuffle, and immediately after the world is returned to normal, Crocker finds the muffin, takes a bite, and wishes he could catch a fairy. He obtains a butterfly net (one of the few items a fairy's magic has no effect on) and managed to kidnap Wanda with it before running off. Distraught, Cosmo attempts to chase after Crocker but Timmy holds him back in fear that he would get captured. Cosmo proceeds to break down in tears and chickens out of the battle. Timmy and Bippy must fight Mr. Crocker themselves, but the Food Cart Guy passes by with more muffins and distracts Bippy, leaving Timmy on his own with a devastated Cosmo. Timmy realizes that the first thing Mr. Crocker will do with his newfound power is rub it in the face of his students, so he rushes back to his school. With his new-found power, Mr. Crocker appears in a power armor suit which has Wanda trapped in a scepter and the Fairy-versary muffin within the chest cavity. He terrorizes his students by turning them into ice statues (he wanted to turn them into ash), turning Sanjay and Chester's heads into toilets when they asked to go the bathroom, and then chases Timmy off as he transforms the world and makes himself the all-powerful magical leader.
Timmy and Cosmo arrive at home before Crocker's magic covers the world. They hide themselves in Santa's magic bag and get unaffected by the magic. Timmy then arms himself with his gifts and goes battle Crocker.
Jorgen Von Strangle scrambles the fairies and destroys the bridge to Fairy World after discovering a fairy has been captured. Crocker is busy making everyone in Dimmsdale, now called Slavesdale, bow down to him, including Chet "Ubowdown", Principal Waxelplax, A.J., Vicky, and Tootie. Timmy shows up and decides to fight magic with magic, and with the help Cosmo, Timmy engages Crocker in a magic sword fight like duel with his magic mirror and Crocker's scepter. The mirror gets broken and Crocker was about to transform Timmy into a shrimp when Cosmo appears "massive pecs" to help defeat Mr. Crocker and free Wanda. Cosmo has the upper hand at first, and nearly defeats Crocker on his own, but he lets his guard down when he is reunited with Wanda and is captured as well. Now on his own, Timmy is transport to space by Crocker, and he uses his magic glasses to get a helmet and breath. Crocker appears and starts attacking, but Timmy throws a rock to him and breaks his glass mask and get transport to ancient Egypt, where Crocker reanimates a Great Sphinx. Timmy uses his Easter eggs on it but it didn't work, so he traps the Sphinx's teeth in floss to control it. Timmy gets advantage but Mr. Crocker destroys the Sphinx's nose, plus getting it back to normal. Crocker now transports everyone to a subatomic level. Timmy hides from Croker but gets capture and remembers what happens when an atom is divided, so he uses his arrow and bow to divide one, causing a giant atomic explosion that bakes everything. Crocker survives via magic and when he thought that he won, Timmy reveals himself alive because he involved in Santa's bag again but his identity is discovered when a cooked bird falls on him and breaks his glasses. Crocker then transports to Timmy's house to intimidate Timmy with his parents. Timmy attaches to Crocker with floss but Crocker throws him to the other end of the portal. Timmy crashes in a part of Slavesdale. The crash gets the Santa's bag unusable and useless but Timmy flies home via jetpack.
Timmy races Crocker back to his ransacked home but is too late, Mr. Crocker has Timmy's parents captured in a magic bubble. Timmy then realizes that there was only one way to fix everything: he yells out loud, with his parents as witnesses, that Cosmo and Wanda are his godparents. As this is the biggest breach of Da Rules, they disappear, and Crocker's power is gone. Now powerless, Mrs. Turner beats up Crocker, knocking out his teeth in the process, as punishment for attacking her family, which causes the muffin to fly out of his hand. Timmy eats it whole and wishes for Cosmo and Wanda to be his godparents again while Mrs. Turner mercilessly knocks out Crocker's teeth and pounds him to a pulp. Timmy's wish is granted, and as they reappear, Timmy wishes - successfully - that everything be returned to normal and that no one remembered he had fairies. Timmy and his godparents secretly watch Mr. Crocker try to claim to people that fairies exist and that they should bow to him. Waxelplax states today Crocker is going to bow down for his medicine. Two guys in hospital gear then take Crocker and hit him with a needle, knocking him out with one guy saying "Nighty-night crazy guy!"
- Ash Ketchum, Pikachu, Misty, Togepi, Brock, The DigiDestined and their Digimon, The Human Mane 5, Littlefoot, Cera, Ducky, Petrie, Spike, Chomper, Ruby, Guido, Grandpa Longneck, Grandma Longneck, Scooby-Doo, Shaggy Rogers, Fred Jones, Daphne Blake, Velma Dinkley, Jafar, Iago, Maleficent, Myotismon, Ursula, Hades, The Grand Duke of Owls, The Dazzlings, The Crime Empire, (Joker, Two-Face, Penguin, Negaduck, Megavolt, Quackerjack, Bushroot, and The Liquidator) and Team Rocket (Jessie, James, and Meowth) guest stars in this film.
- Like Pooh's Adventures of The Fairly Oddparents: Abra-Catastrophe The Movie, Jafar, Iago, Maleficent, Myotismon, Ursula, Hades, The Grand Duke of Owls, The Dazzlings, The Crime Empire and Team Rocket will work for Denzel Crocker.
- This film take places after Pooh and Ash's Adventures of Scooby-Doo and the Headless Horseman of Halloween (Witch explains that Timmy Turner, Cosmo and Wanda already faced Jafar, Iago, Maleficent, Myotismon, Ursula, Hades, The Grand Duke of Owls before).
- Dr. Facilier, Winnie the Pooh, and Batman were mentioned in this film.
- The DigiDestined and their Digimon will meet Timmy Turner, Cosmo and Wanda for the first time.
- Timmy Turner, Cosmo and Wanda will meet The Dazzlings, The Crime Empire and Team Rocket for the first time.
- Timmy Turner, Cosmo and Wanda will see Sunset Shimmer again in Pooh's Adventures of The Fairly OddParents: Channel Chasers.
- Dani Marcus - Open my Eyes (Ending Credits)