Late in the night, 5 years old Princess Anna wakes her elder sister, 8 years old Princess Elsa to play using Elsa's snow magic. However when the magic goes awry, the royal family journeys into the wilderness to seek the help of trolls who remove the magic from Anna, and her memory of her sister's power. In order to protect Elsa from the world, she is ostracized from everyone, including Anna, leaving both sisters distraught and lonely. Their despair only escalates when their parents die after they go on a ship to sail somewhere when a storm erupts and a wave capsizes the boat.
Three years later, it is the day of Elsa's (Idina Menzel) coronation ceremony. Dignitaries from around the world are coming to visit, including the Duke of Weselton (Alan Tudyk), who wants to run Arendelle's profits dry. Nobody is more excited than Anna (Kristen Bell), as they are finally opening the gates to the kingdom. She is happy to see other people, and hopes for the possibility of meeting that special someone, but Elsa is still concerned about trying to control her powers. As she strolls out onto the streets, she bumps into a horse, which happens to belong to the charming and handsome Prince Hans (Santino Fontana) of the Southern Isles. Anna is instantly attracted to him. The coronation goes off without a hitch, despite Elsa's fears and she even takes time to connect with her sister at the party before Anna and Hans sneak off to spend the evening together, quickly realizing the mutual attraction between them. Hans proposes right on the spot, and Anna immediately accepts. Elsa refuses to grant her blessing on the marriage, setting off an argument between the sisters culminating in Elsa's powers being exposed to the party guests.
Panicking, Elsa runs with Anna in hot pursuit. As she becomes more stressed and panicked, the weather starts turning colder: snow begins to fall and Elsa races across the fjord, freezing it with each step. She makes it to the North Mountain where she laments her failure at keeping the powers contained but quickly becomes more and more at ease and relaxed, free to use her powers as she pleases building a snow man, an ice palace, and an ice dress.The next morning Anna is on a hunt for Elsa, determined to return her to Arendelle, end the winter, and mend their relationship. She finds herself at Oaken's Trading Post where she meets Oaken (Chris Williams) and Kristoff (Jonathan Groff). Anna convinces Kristoff to take her to the North Mountain where the source of the winter is coming from. After an attack from wolves the pair meet the snowman Elsa created, named Olaf (Josh Gad) who shares his dreams of experiencing summer and agrees to lead them to Elsa's hideout.
The gang makes it to Elsa's palace. Anna and Elsa reunite, and while both are happy to see each other, Elsa still harbors fears of wounding Anna once again. Despite Anna's promising to stand by her sister and help her, Elsa only grows more agitated and nervous resulting in her magic flaring, this time striking Anna in the heart. Elsa, in desperation to get her sister to safety, creates a giant snow creature (that Olaf calls "Marshmallow") to throw them out. After they outrun the snow monster, Kristoff notices that Anna's hair is turning white, and he takes them to seek help. Kristoff leads Anna (who is slowly freezing) to the trolls. After a mix-up where the trolls insist Anna and Kristoff get married, she collapses and Grand Pabbie appears. According to him, only an act of true love can thaw a frozen heart or else Anna will freeze solid. Kristoff races back to Arendelle to get Anna to Hans, believing true love's kiss will save her.
Meanwhile, Hans, on a search for Anna after her horse returns to the kingdom without her, and the guards find the ice castle. While Hans battles Marshmallow the duke's men attack Elsa who fights back, nearly killing them both much to her own horror. At the end of the altercation Elsa's chandelier comes crashing down, knocking her unconscious. She wakes up shackled in a cell back in Arendelle. Hans pleads with her to undo the winter to no avail but Elsa replies that she can't due to the fact that she is unable to control her powers. Anna is returned to Hans, freezing more and more by the minute. She begs him to kiss her and break the curse but he refuses, revealing his plan all along was to marry into Arendelle's throne, because he is the youngest of 12 brothers and he will never reach the throne. He leaves Anna to freeze to death and goes to kill Elsa. Also, he puts the fire out to prevent Anna from getting any warmth.
Elsa escapes from prison when she learns Hans means to have her executed for treason and heads out into the blizzard on the fjord. In the castle, Olaf comes to Anna's rescue and reveals Kristoff is in love with her. The two then travel together out on the fjord to find him, where he is racing back to the kingdom. Hans cuts Elsa off and tells her Anna is dead because of her. In Elsa's despair, the storm immediately stops, giving Kristoff and Anna the chance to reach each other. But when Anna sees Hans is about to kill her sister, she chooses instead to save Elsa, throwing herself between Elsa and Hans; she freezes solid just as Hans' sword hits her instead of Elsa. After a few moments of despair, Elsa sees her sister has thawed and come back to life because she sacrificed herself to save her sister, constituting an act of true love.
Elsa realizes love is the key to controlling her powers and thaws the kingdom. Sometime later, Anna gifts Kristoff a new sled and reveals Elsa has named him the Official Ice Master for the kingdom and the two share a kiss. Hans is sent back to his own kingdom in chains where he will have to deal with his 12 older brothers for his deeds and Elsa cuts off trade with Weselton. Elsa promises to never shut the castle gates again and skates on the new ice rink with Anna.
- Ash Ketchum, Pikachu, Misty, Togepi, Brock, SpongeBob SquarePants, Patrick Star, Ed, Edd, Eddy, The DigiDestined and their Digimon, The Masters of Evil, and The Crime Empire (Joker, Harley Quinn, Two-Face, The Penguin, and the Fearsome Five) will guest star in this film.
- The film features the first debut of Rocky and Bullwinkle, the Clone Troopers (once Stormtroopers, freed from Order 66 w/ Order 51 (reversed the effects of Order 66)), Jack Skellington, Zero, the Teenage Mutant Ninja Turtles, Splinter, Pit, Lady Palutena (revealed to be the Good Fairy's mother), the Mighty Morphin Power Rangers, Zordon, Alpha 5, Ninjor, Ahsoka Tano (from Star Wars: The Clone Wars), Robo Knight, N, Anthea and Concordia, and The Masters of Evil.
- The Masters of Evil in this film include: King Bowser, Bowser Jr., Mistress Nine, Azula (from Avatar: The Last Airbender), The Psycho Rangers, The Phantom Blot, Dr. Eggman, Orbot and Cubot, Metal Sonic, King Ghidorah, SpaceGodzilla, Cyber-Godzilla, Team Rocket (Jessie, James, and Meowth), Giovanni, Sheldon J. Plankton, Mercurymon, Ranamon, Grumblemon, and Arbormon, Lord Zedd, Rita Repulsa, Goldar, Scorpina, Rito Revolto, and Master Vile (from Might Morphin Power Rangers), The Machine Empire (from Power Rangers Zeo), Oogie Boogie, Lock, Shock, and Barrel, The Dark Masters (from Digimon), Cat R. Waul (from An American Tail: Fievel Goes West), Count Dooku (clone with memories of the original), Emperor Palpatine (Darth Sidious) (clone with memories of the original), Lord Dragaunus, Siege, Chameleon, and Wraith (from the animated Mighty Ducks TV series), Fearless Leader, Boris Badenov, Natasha Fatale, and Vrak and his Zombats.
- This film is the first Winnie the Pooh crossover to feature the Teenage Mutant Ninja Turtles since Bowser's original version of Pooh's Adventures of Space Jam.
- This is the second Winnie the Pooh crossover with a recently released Walt Disney Animation Studios film, the first being Winnie the Pooh Meets The Princess and the Frog.
- This is the third time that Pooh and his friends meet another character named Elsa; the first two being Pooh's Adventures of We're Back! A Dinosaur's Story and Pooh's Adventures of Frankenweenie.
- Elsa the Snow Queen and Robo Knight join Pooh and his friends on their next adventures at the end of this film.
- BrerJake90 was originally going to make this film, but he retired from making anymore Pooh's Adventures films, so BowserMovies1989 will make this film instead.
- The Winnie the Pooh series, The Nightmare Before Christmas, Mighty Ducks, Darkwing Duck, and Frozen were all made by Disney.
- The Masters of Evil, and The Crime Empire will be working with Prince Hans in this movie.
- Both Winnie the Pooh and Frozen were produced by Walt Disney Animation Studios and featured songs composed by Robert Lopez and Kristen Anderson-Lopez.
- The film takes place before Billy2009's Winnie the Pooh vs The Mummy.
- In the epilogue Lysandre the boss of Team Flare made his appearance is where he, his grunts, scientists and the villains freed Prince Hans from being locked away in Southern Isle and became impressed of Prince Hans' villainous deeds, and he made a deal with him to do all his work. Prince Hans will soon get his revenge and kill his mortal enemy is Ash Ketchum along with Pooh and his friends in TMNTSubspace's TV show Team Robot In Pokemon XY The Series.